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12-11a). If the gradient with sample layered over is positioned in the inclined situation in the rotor, the sample zone and equal density planes in the gradient form ellipses.
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These drawbacks will not be severe in a few instances, since the big subcellular particles of a tissue homogenate have sedimentation coefficients that vary from each other by a number of orders of magnitude.
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Differential centrifugation has quite a few main disadvantages. As the homogenate is initially distributed uniformly all over the centrifuge tube, the first particles sedimented will automatically be contaminated with compact quantities of all other constituents on the homogenate. This result is shown in Figure twelve-five.
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